Journal: Scientific Reports

Article Title: CD1 − and CD1 + porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

doi: 10.1038/srep40942

Figure Lengend Snippet: Expression of MHC class II DR and CD80/86 by monocytes, CD1 − cDC, CD1 + cDC and pDC upon isolation (fresh) or following an 18 h culture (cultured) was assessed by flow cytometry. MHC class II DR and CD80/86 staining (black histograms) was compared against the corresponding isotype control antibody (grey histograms) and representative data is shown from 1 of 3 individual pigs analysed.

Article Snippet: The sorted DC subsets and monocytes were seeded at 5 × 10 4 /well and stained with 10 μl of porcine MHC class II-DR (IgG1, clone 2E9/13), CD11R3 (CD11b-like; IgG1 clone 2F4/11), CD16 (IgG1, clone G7) mAbs (all Bio-Rad, Oxford, UK) and huCD152-muIg fusion protein (IgG2a, Enzo Life Sciences, Exeter, UK) conjugated to R-PE using Zenon ® Mouse IgG Labelling Kits (Life Technologies), and with biotinylated polyclonal anti-huCD83 antibody (R&D Systems, Abingdon, UK).

Techniques: Expressing, Isolation, Cell Culture, Flow Cytometry, Staining, Control